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. 2019 Oct 10;20(12):e47728. doi: 10.15252/embr.201947728

Figure 7. MITOL translocation to peroxisomes is mediated by peroxins.

Figure 7

  1. siRNA‐based knockdown of Pex3, Pex16, and Pex19. HeLa cell lysates treated with the indicated plasmids and siRNAs were immunoblotted with the indicated antibodies.
  2. A comparable amount of MITOL was detected in control, PEX3‐, PEX16‐, and PEX19‐knockdown cells. HeLa cell lysates stably expressing HA‐Parkin and 3Flag‐MITOL treated with the corresponding siRNAs were immunoblotted with the indicated antibodies.
  3. MITOL translocation from the mitochondria to peroxisomes is highly dependent on Pex3. HeLa cells stably expressing 3Flag‐MITOL and HA‐Parkin were transfected with control, PEX3, PEX16, or PEX19 siRNA, treated with 15 μM CCCP for 3 h, and then subjected to immunocytochemistry with anti‐Flag and anti‐catalase antibodies. Scale bars, 10 μm.
  4. Correlation statistics for the localization of 3Flag‐MITOL and catalase in cells transfected with the indicated siRNAs. Dots indicate individual Pearson correlation coefficient data points. In the box‐plots, the center lines indicate the medians, the box limits indicate the 25th and 75th percentiles as determined in the R software package, and the whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Means and the number of samples are shown on the box and X‐axis, respectively. Statistical significance was calculated using a one‐tailed Welch's t‐test.