Skip to main content
. 2019 Oct 21;213(4):1387–1400. doi: 10.1534/genetics.119.302617

Figure 2.

Figure 2

NCU05712 (rcd-1) is necessary and sufficient for GRD induction in N. crassa. (A) A flow cytometry-based quantification of GRD using the vital dye PI (Heller et al. 2018) shows that rcd-1 is necessary for the GRD phenotype. (B) GRD quantification with isogenic strains of N. crassa carrying different rcd-1 alleles. Strains carrying ectopic rcd-1 allele at the his-3 locus are indicated with (h::) preceding the allele. Experiments were performed in triplicate, with 20,000 events counted per experiment. *P < 0.0006, one-way ANOVA with Tukey’s multiple comparisons test. (C) Reconstitution of rcd-1-1/rcd-1-2 GRD phenotype in isogenic N. crassa strains. Cells undergoing GRD (white arrows) show strong vacuolization and uptake of PI. Deletion of rcd-1 abolishes GRD and genetically distinct strains can undergo cell fusion to form viable cell networks. Fusion points between genetically distinct germlings are shown with black asterisks. Bar, 5 µm.