Figure 5.

Adolescent stress differentially affects DVB presynaptic vesicle cluster morphology. (A) Confocal z-stack maximum projections of DVB neuron and DVB presynaptic puncta (lim-6^int4::gfp::rab-3) in fed control and mid-L4 stressed males at day 1 of adulthood (arrowheads indicate DVB neurites with little/no gfp or diffuse gfp). Bar, 10 μm. Identically thresholded z-stack maximum projections of the gfp::rab-3 channel created particle outlines of synaptic puncta on DVB neurites for analysis of intensity and other measures. Quantification of lim-6^int4::gfp::rab-3 (B) average particle area and (C) average mean intensity in control and mid-L4 stressed males at day 1.