Figure 1.

The sexually dimorphic expression of Amh in early embryonic gonads of P. sinensis. (A and B) The transcript expression levels of Amh in gonads of both sexes during the critical sex determination period (stages 15–19), determined by RNA-sequencing (A) and qRT-PCR (B). Amh exhibited a highly male-specific expression pattern in early embryonic gonads. Data are shown as means ± SD. N ≥ 3. (C) Immunofluorescence of AMH in male and female embryonic gonads at stage 17. AMH protein was robustly expressed in the medullary region of ZZ gonads. Bar, 50 μm.