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. 2019 Oct 23;213(4):1317–1327. doi: 10.1534/genetics.119.302527

Figure 2.

Figure 2

Upregulation of Amh in masculinized ZW gonads induced by aromatase inhibitor (AI). (A) Tail morphology (black arrow) and hematoxylin and eosin (H&E) staining of gonadal sections from control ZW, ZW + AI, and control ZZ P. sinensis of stage 27. The male-to-female sex reversal were observed in ZW + AI gonads characterized by morphologically altered tail and medullary sex-cord formation. Bar, 5 mm and 50 μm, respectively. (B) Double immunofluorescence of SOX9 and CTNNB1 in gonadal sections of control ZW, ZW + AI, and control ZZ P. sinensis of stage 27. Ectopic expression of SOX9 protein were activated in masculinized medulla of ZW gonads. Bar, 50 μm. (C) The mRNA expression of Amh in ZW gonads with AI treatment at stage 17, 21, and 25, showing rapid and remarkable upregulation, determined by qRT-PCR analysis. Data are shown as means ± SD. N ≥ 3. ** P < 0.01; *** P < 0.001. cor, cortical region; med, medullary region; pf, primordial follicle; sc, Sertoli cell.