Masculinization of ZW embryos overexpressing Amh in ovo. The tails (A–C), GMCs (D–F), and H&E staining of gonadal sections (G–I) from control ZW, ZW + Amh-OE, and control ZZ embryos. The ZW embryos overexpressing Amh showed the female-to-male sex reversal, characterized by curved tails and male-like gonads with seminiferous cord-like structure in medulla. Gonads were outlined by yellow dotted lines. Bar, 1 cm (A–C), 1 mm (D–F), and 50 μm (G–I), respectively. cor, cortical region; gc, germ cells; Gd, gonad; med, medullary region; Ms, mesonephros; sc, Sertoli cell.