Table 1. Phenotypes of embryos after LV-Amh-shRNA or LV-Amh-OE treatment.
| Viral treatment | No. of injected embryos | Embryos surviving until stage 25 | Genotype of embryos | Phenotype of embryos | Sex reversal ratea |
|---|---|---|---|---|---|
| LV-NC-shRNA | 200 | 175 | ZZ:88; ZW:87 | M:88; F:87 | 0/88 |
| LV-Amh-shRNA | 200 | 138 | ZZ:64; ZW:74 | M:43; F:95 | 21/64 |
| LV-empty | 200 | 150 | ZZ:78; ZW:72 | M:78; F:72 | 0/72 |
| LV-Amh-OE | 200 | 124 | ZZ:62; ZW:62 | M:78; F:46 | 16/62 |
Genotype of embryos was identified by amplification of sex chromosome-specific DNA fragment. Phenotype of embryos was assessed by gonadal histology and SOX9 immunofluorescence.
a
Male-to-female sex reversal rate was determined by the number of feminized genetic male embryos divided by the total number of ZZ embryos; female-to-male sex reversal rate was determined by the number of masculinized genetic female embryos divided by the total number of ZW embryos.