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. 2019 Oct 14;213(4):1289–1299. doi: 10.1534/genetics.119.302583

Figure 4.

Figure 4

Quantitative RT-PCR analysis of GFP transcript levels generated by GMR activity in cis and trans. (A) Total RNA from whole third instar larval eye-antennal discs of each genotype was subjected to quantitative RT-PCR using GFP-specific primers. All data are presented as relative to cis-expression from position 53F (= 1.0). Each bar in the figure represents three biological replicates; error bars represent 95% confidence interval. Dotted box highlights exceptional trans-activity at position 96C. (B) GFP expression by GMR as assessed by qRT-PCR (y-axis; data from (A)) vs. mean fluorescence (x-axis; data from Figure 2 and Figure 3; a.u., arbitrary units). Outliers with evidence of ectopic position-dependent GFP mRNA outside of the GMR expression domain are indicated (37C, 96F). (C) qRT-PCR using GFP-specific primers demonstrating elevated GFP RNA when GMR acts in trans at position 65E (enhancerless/promoterless) relative to the enhancerless construct in the absence of GMR at that position.