Table 1.
Primers used in reverse transcriptase–polymerase chain-reaction analysis.
| Gene Name | Sequence | Sequence | ||
|---|---|---|---|---|
| PPAR-𝛾 | Forward | 5-GAA AGA CAA CGG ACA AAT CAC-3 | Reverse | 5-GAA ACT GGC ACC CTT GAA-3 |
| C/EBP-𝛼 | Forward | 5-CGT CTA AGA TGA GGG AGT C-3 | Reverse | 5-GGC ACA AGG TTA CTT CCT-3 |
| SREBP−1c | Forward | 5-CTT CTG GAG ACA TCG CAA AC-3 | Reverse | 5-GGT AGA CAA CAG CCG CAT C-3 |
| ACS | Forward | 5-AAG CCC AGA GTT ACG AGT AT-3 | Reverse | 5-ACA CAG GAA TAG AGG AGT TCT-3 |
| FAS | Forward | 5-CTT GGG TGC TGA CTA CAA CC-3 | Reverse | 5-GCC CTC CCG TAC ACT CAC TC-3 |
| HMGCR | Forward | 5-TTC TGC TCT TGA TTG ACC TTT C-3 | Reverse | 5-TTT CCC TTA CTT CAT CCT GTG A-3 |
| SIRT1 | Forward | 5-GGC GGG GAA CGA CTG CG-3 | Reverse | 5-GGA GTC ATG GGG GCT GTA CTG-3 |
| TNF-α | Forward | 5-AAG CCT GTA GCC CAC GTC GT-3 | Reverse | 5-GGC ACC ACT AGT TGG TTG TC-3 |
| IL-1β | Forward | 5-AAC CAA GCA ACG AVA AAA TA-3 | Reverse | 5-AGG TGC TGA TGT ACC AGT TG-3 |
| IL-6 | Forward | 5-CCG GAG AGG AGA CTT CAC AG-3 | Reverse | 5-GGA AAT TGG GGT AGG AAG GA-3 |
| IL-10 | Forward | 5-TCA GCT GTG TCT GGG CCA CT-3 | Reverse | 5-TTA TGA GTA GGG ACA GGA AG-3 |
| MCP-1 | Forward | 5-TGA TCC CAA TGA GTA GGC TGG AG-3 | Reverse | 5-ATG TCT GGA CCC ATT CCT TCT TG-3 |
| PGC-1α | Forward | 5-ATT CGG GAG CTG GAT GGC TT-3 | Reverse | 5-CCG ATT GGT CGC TAC ACC AC-3 |
| UCP3 | Forward | 5-ACC CGA TAC ATG AAC GCT CC-3 | Reverse | 5-TCA TCA CGT TCC AAG CTC CC-3 |
| CPT-1 | Forward | 5-TGT GTG AGG ATG CTG CTT CC-3 | Reverse | 5-CTC GGA GAG CTA AGC TTG TC-3 |
| Nrf2 | Forward | 5-AGC ACA TCC AGA CAG ACA CCA GT-3 | Reverse | 5-TTC AGC GTG GCT GGG GAT AT-3 |
| SOD | Forward | 5-CAA TGG TGG GGG ACA TAT TA-3 | Reverse | 5-TTG ATA GCC TCC AGC AAC TC-3 |
| CAT | Forward | 5-GAA CGA GGA GGA GAG GAA AC-3 | Reverse | 5-TGA AAT TCT TGA CCG CTT TC-3 |
| GPx | Forward | 5-ACA TTC CCA GTC ATT CTA CC-3 | Reverse | 5-TTC AAG CAG GCA GAT ACG-3 |
| GR | Forward | 5-CGG CGA TCT CCA CAG CAA TG-3 | Reverse | 5-ACC GCT CCA CAC ATC CTG ATT G-3 |
| GAPDH | Forward | 5-GCA CAG TCA AGG CCG AGA AT-3 | Reverse | 5-GCC TTC TCC ATG GTG GTG AA-3 |
PPAR-𝛾, peroxisome proliferator-activated receptor γ; C/EBP-𝛼, CCAAT/enhancer binding protein α; SREBP−1c, sterol regulatory element-binding protein 1c; ACS, acyl-CoA synthetase, FAS, fatty acid synthase; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; SIRT1, sirtuin 1; TNF-α, tumor necrosis factor-α; Il-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; MCP-1, monocyte chemoattractant protein-1; PGC-1α, peroxisome proliferator-activated receptor-gamma coactivator 1-alpha; UCP3, uncoupling protein 3; CPT-1, carnitine palmitoyltransferase I; Nrf2, nuclear factor erythroid-derived 2-related factor 2; SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase; GR, glutathione reductase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.