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Anti-inflammatory effect of PU on LPS-induced RAW264.7 macrophages. (A–C) Cells were pre-treated with various concentrations of PU for 1 h and treated with LPS for an additional 24 h. The NO content was determined by Griess reagent and the production of cytokines were measured by enzyme-linked immunosorbent assay (ELISA) kit using the microplate reader. The data are presented as means ± SD (n = 3). (*, p < 0.05 and ** p < 0.01 vs. LPS group, and ##, p < 0.001 vs. control group).
