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Effects of PU (A) on LPS-induced Foxo3a in RAW264.7 macrophages. Cells were pre-treated with PU (12.5–50 μM) for 1 h before exposure to LPS (1 μg/mL) for 30 minutes. Representative image from three independent experiments are shown along with β-actin as the internal loading control. The data are presented as means ± SD (n = 3). (**, p < 0.01 vs. LPS group; and ##, p < 0.01 vs. control group).
