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. 2019 Oct 31;11(11):1009. doi: 10.3390/v11111009

Figure 4.

Figure 4

C4 affects some, but not all, BRI1 and FLS2 downstream readouts. Relative expression of the BL responsive gene EXP8 (A) or of the flg22 responsive genes FRK1 and CYP8IF2 (B). Bars represent mean expression in 10-day-old T3 35S:GFP and T2 35S:C4 (L5) transgenic Arabidopsis seedlings treated with 1 µM epiBL (A) or 1 µM flg22 (B) one hour prior sample harvesting ± SD, n = 3 (technical replicates), as measured by quantitative real-time-polymerase chain reaction (qRT-PCR). Three independent biological replicates were performed, and results from one representative replicate are shown. (C,D) Production of reactive oxygen species (ROS) in four-week-old wild-type (WT) and T3 35S:C4 transgenic Arabidopsis (L5, L7) plants upon 100 nM flg22 treatment measured as total photon counts during 60 min (C) (bars represent averages values ± SE, n = 12) or as relative luminescence units (RLU) along 40 min (D). Three independent biological replicates were performed; values correspond to one representative replicate ± SE, n = 12 (leaf discs from independent leaves). (E) Normalized root length of 12-day-old T3 35S:GFP and T2 35S:C4 (L5) transgenic Arabidopsis seedlings following depletion of (2 µM Propiconazol (PPZ)) and exogenous addition of brassinosteroids (0.1 and 1 nM epiBL) for six days, ±SD, n = 12–16. Three independent biological replicates were performed with similar results; results from one replicate are shown. (F) Relative seedling growth inhibition of 10-day-old WT and T3 35S:C4 transgenic Arabidopsis (L7, L5) seedlings upon 100 nM flg22 treatment ± SE, n = 14–16. Three independent biological replicates were performed with similar results; results from one replicate are shown.