Effects of ajoene extract on myokines secretion in CT26 tumor-bearing mice. (A,B) mRNA expression of interleukin-6 (IL-6), interleukin-6 receptor (IL-6R), and myostatin in the quadriceps muscle; (C) representative contour plots showing the proportions of macrophages (top) and MDSCs (bottom) in the spleen. Values are presented as mean ± SEM. Statistical significance was evaluated by one-way ANOVA followed by the Duncan’s multiple range test or Student’s t-test. Means with different superscript letters are significantly different at p < 0.05 (A) and p < 0.001 (B). * p < 0.05 compared with the C group; # p < 0.05 and ## p < 0.01 compared with the TC group. MDSCs, myeloid-derived suppressor cells; C, control; TC, tumor control; A5, 5 mg/kg ajoene extract; A10, 10 mg/kg ajoene extract (n = 5 per group).