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. 2019 Nov 10;11(11):2724. doi: 10.3390/nu11112724

Figure 4.

Figure 4

Effect of Z-ajoene on myoblast differentiation. (A) Structure of Z-ajoene. (B) C2C12 myoblasts were differentiated using differentiation medium (DM) supplementary with Z-ajoene (0.1, 1, 10, or 100 nM) for 3 days and then collected for immunostaining of myosin heavy chain (MHC) (red) and 4’,6-diamidino-2-phenylindole (DAPI, blue). Scale bar = 200 μm. (C) Differentiated C2C12 cells in the presence of Z-ajoene were subjected to Western blot analysis to determine the expression level of MHC. (D) Day-dependent effect of Z-ajoene (100 nM) on expression levels of myogenic factors during myoblast differentiation.