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Effect of Z-ajoene on p38 MAPK activation during myoblast differentiation. (A) C2C12 cells were treated with Z-ajoene (100 nM) during days 1–3 of differentiation (D1–D3). Collected cell lysates were subjected to immunoblot. (B) C2C12 cells were pre-treated with (+) or without (-) SB203580 (10 μM) prior to Z-ajoene and differentiated in DM for 2 days. Cell lysates were subjected to Western blot analysis and immunostained with MHC (red) and DAPI (blue). Scale bar = 200 μm.
