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. 2019 Nov 28;7:822. doi: 10.3389/fchem.2019.00822

Figure 4.

Figure 4

FP-based HTS of the ChemBridge 50K compound diversity set with the Hck-U32L protein identifies four hit compounds with a shared pyrimidine diamine substructure. The structures of the four hit compounds are shown at the top. Each compound was tested for direct interaction with each of the Hck and Fgr proteins listed using SPR as described in the legend to Figure 5. SPR data were best fit by a 1:1 Langmuir model yielding the kinetic KD values shown ± S.E. No binding was observed with the isolated Hck SH3 or SH2 domains. Peptides that selectively interact with the SH3 domain (VSL12) or the SH2 domain (pYEEI) were included as positive controls. NI, no interaction; n.d., not determined.