Figure 3.

α-GalCer-induced iNKT cell activation and proliferation are suppressed by α-LacCer in vivo. BALB/c mice were injected intraperitoneally with 1 μg of α-GalCer and α-LacCer, and the lungs were harvested 3 days post-injection. (A) Flow cytometry analysis of CD69 and Ki67 expressions in iNKT cells (CD45⁺TCRβ⁺CD1d tetramer⁺ cells). (B) Absolute numbers of total, CD69⁺, and Ki67⁺ iNKT cells calculated from the flow cytometry data (n = 3). (C) IFN-γ and IL-4 levels in the lung lysates were measured by ELISA (n = 3). Data are representative of three independent experiments and presented as means ± s.e.m. [^**P < 0.01 and ^***P < 0.001, Student's t-test (B,C)].