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. 2019 Nov 16;11(11):2796. doi: 10.3390/nu11112796

Figure 2.

Figure 2

Liver superoxide anion production and antioxidant defense protein expression in the NF, NFCR, OF, and OFCR groups at seven months of age. Superoxide anion production was measured by hydroethidine staining (A) at the same magnification (20×) in the NF, NFCR, OF, and OFCR groups. Nuclei were counterstained with DAPI. A negative control was included. Hydroethidine fluorescence intensity was quantified using ImageJ (B). ***p < 0.001, N.S: nonsignificant, p > 0.05. These pictures are representative images from n = 5 animals/group. Cu/Zn superoxide dismutase (Cu/Zn Sod) (C,D) and catalase (E,F) protein expression levels were measured by western blot in the OF and OFCR groups. Cu/Zn superoxide dismutase (Cu/Zn Sod) (G,H) and catalase (I,J) protein expression levels were also measured by western blot and in the NF and NFCR groups. Values are reported as the mean ± SD; (*) p < 0.05; N.S: nonsignificant, p > 0.05; n = 5–4. animals/group.