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. 2019 Nov 10;11(11):1045. doi: 10.3390/v11111045

Figure 4.

Figure 4

Rg1 suppresses inflammatory cytokines mRNA expression in infected PAMs and Marc-145 cells. (A) For PRRSV+Rg1 group, the XH-GD strain (0.1 MOI) infected Marc-145 cells for 1 h and then cultured in DMEM supplemented with Rg1 (200 μM), and cells infected with virus or treated with Rg1 (200 μM) were termed as PRRSV group and Rg1 group, respectively. Cells in the mock group were grown in DMEM containing 0.4% DMSO. Cell samples were collected to extract total RNA at 12, 18, and 24 h.p.i. The relative expression of IL-6, IL-8, IL-1β and TNF-α was analyzed by RT-PCR. GAPDH was used as internal control to normalize values. (B) PAMs were cultured with RPMI 1640 and treated as described above. The data of each trial represents three independent experiments and the values are shown as the means ± SD. T-test was applied to perform statistical analysis and the significance was indicated by asterisk in the graphs. Statistical significance is denoted by * p < 0.05, ** p < 0.01, and *** p < 0.001.