Table 1.
Sequences of the primers and probe used for Real-time polymerase chain reaction (PCR).
| Name | Primer Sequence (5′-3′) |
|---|---|
| PRRSV Nsp9 | F: CCTGCAATTGTCCGCTGGTTTG |
| R: GACGACAGGCCACCTCTCTTAG | |
| GAPDH | F: GCAAAGACTGAACCCACTAATT |
| R: TTGCCTCTGTTGTTACTTGGAG | |
| Marc-145 IL-6 | F: GAGGCACTGGCAGAAAAC |
| R: TGCAGGAACTGGATCAGGAC | |
| PAM IL-6 | F: CCTTCAGTCCAGTCGCCTTCTC |
| R: CATCACCTTTGGCATCTTCTTC | |
| Marc-145 IL-8 | F: AGGACAAGAGCCAGGAAG |
| R: CTGCACCTTCACACAGAGC | |
| PAM IL-8 | F: CACTGTGAAAATTCAGAAATCATTGT |
| R: CTTCACAAATACCTGCACAACC | |
| Marc-145 TNF-α | F: TCTGTCTGCTGCACTTTGGAGTG |
| R: TTGAGGGTTTGCTACAACATGG | |
| PAM TNFα | F: TGGTGGTGCCGACAGATGG |
| R: GGCTGATGGTGTGAGTGAGG | |
| Marc-145 IL-1β | F: GGAAGACAAATTGCATGG |
| R: CCCAACTGGTACATCAGC | |
| PAM IL-1β | F: ACCTGGACCTTGGTTCTCTG |
| R: CATCTGCCTGATGCTCTTG | |
| Probe JXA1 Nsp9 | ACTGCTGCCACGACTTACTGGTCACGCAGT |
F: forward primer; R: reverse primer.