Skip to main content
. 2019 Oct 27;11(11):2587. doi: 10.3390/nu11112587

Figure 2.

Figure 2

Figure 2

Effects of treatment with synthetic gliadin peptides or wheat gluten hydrolysate (WGH) on reactive oxygen species (ROS) production in adult-stage C. elegans worms. (A) Schematic of α-gliadin motifs with respective peptide sequences. Three kinds of synthetic α-gliadin peptides were generated that either possess cytotoxic activity (red) or gut-permeating activity (green or blue) as previously reported [7]. (B,C) Wild-type N2 animal populations synchronized at the L4 larval stage were treated with synthetic gliadin peptides and ROS production was measured by 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) staining 24 h after treatment. Pictures show representative images of DCFDA staining indicating ROS production after treatment (B). Bar graph (C) shows the pixel intensities from DCFDA fluorescence per worm. Error bars represent s.d.; n.s., not significant; ** p < 0.005 (Student’s t-test). (D,E) Wild-type N2 animal populations synchronized at the L4 larval stage were treated with WGH and ROS production measured by DCFDA staining after 24 h of treatment. Pictures show representative images by DCFDA staining indicating ROS production after treatment (D). Bar graph (E) shows the pixel intensities from DCFDA fluorescence per worm. Error bars represent s.d. n.s., not significant. (Student’s t-test).