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. 2019 Oct 27;11(11):2587. doi: 10.3390/nu11112587

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of the synthetic gliadin peptide GP151–170 on reactive oxygen species (ROS) production in mammalian cell cultures. (A,B) Cell viability in RAW264.7 macrophages after treatment with either the synthetic gliadin peptide GP110–130 or GP151–170. (C) Intracellular ROS levels were evaluated by measuring the intensity of 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence using a microplate reader. A bar graph showing the pixel intensities of DCFDA fluorescence is shown. Error bars represent s.d. ** p < 0.01. *** p < 0.001 (Student’s t-test). C, control.

Effects of the synthetic gliadin peptide GP151–170 on reactive oxygen species (ROS) production in mammalian cell cultures. (A,B) Cell viability in RAW264.7 macrophages after treatment with either the synthetic gliadin peptide GP110–130 or GP151–170. (C) Intracellular ROS levels were evaluated by measuring the intensity of 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence using a microplate reader. A bar graph showing the pixel intensities of DCFDA fluorescence is shown. Error bars represent s.d. ** p < 0.01. *** p < 0.001 (Student’s t-test). C, control.