Figure 9.

cep-1 and mpk-1 function in different tissues is required for increases in germ cell apoptosis triggered by gliadin intake. (A,B) After RNA interference (RNAi) treatment of control, cep-1, or mpk-1 worms at the L1 stage, worms were grown in nematode growth medium (NGM) plates and treated with or without gliadin for starting at the L4 stage for 24 h. Acridine orange (AO)-positive germ cells were observed in either RNAi-treated rrf-1 or ppw-1 mutant backgrounds 24 h after gliadin treatment. n.s., not significant. * p < 0.05. ** p < 0.005 (Student’s t-test).