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. 2019 Nov 6;11(11):1031. doi: 10.3390/v11111031

Figure 3.

Figure 3

Replication kinetics and infectious virus production in A549 cells. (A,B) A549 cells were infected with clinical isolates and RSV reference strains A2 and B1. Cultures were fixed after 24 h, 48 h and 72 h, permeabilized and stained with pAb goat-anti-RSV antibody and AF488 donkey-anti-goat (IgG). Nuclei were visualized with DAPI and cultures were analysed with fluorescence microscopy. RSV positive cells were counted and calculated to the total number of nuclei to reach a percentage of RSV infected cells. (A) Replication kinetics of RSV-A clinical isolates and (B) Replication kinetics of RSV-B clinical isolates. (C,D) A549 cells were infected with clinical isolates and RSV reference strains A2 and B1. After 24 h, 48 h and 72 h, supernatants were collected and used for quantification by conventional plaque assay. Data represents mean values ± SEM (n = 3), significant differences compared to the reference strains are indicated by *p < 0.05; ***p < 0.001 (two-way ANOVA).