Figure 8.

mRNA levels of mucins 1, 4, 5AC and 5B in infected A549 cells. A549 cells were infected with a MOI of 0.1 of clinical isolates and reference strains for 2 h at 37 °C. Inoculum was replaced with DMEM-10 and cells were incubated for 48h at 37 °C. Afterwards, cells were lysed, total RNA was extracted and the expression of MUC1 (A), MUC4 (B), MUC5AC (C) and MUC5B (D) was determined by qRT-PCR. Data represents mean values ± SEM (n = 3), statistically significant differences compared to the reference strains are indicated with ***p < 0.001 (one-way ANOVA).