Table 2.
qPCR primer details.
| Gene | Accession no. | Forward (5’-3’) | Reverse (3’-5’) | Product (bp) |
|---|---|---|---|---|
| HMGR | XM_003461336.3 | TACAGACATGGGCATTGG GT | GGCAGGGAAAGTGTTGAGTG | 184 |
| LDLR | XM_013149927.1 | GACGTGTCCCAGAGG AAGAT | CGAGTCGGTCCAGTAGATGTT | 144 |
| SREBP2 | XM_003470391.3 | GGGGCTCAAAGGTCTTCTCT | AGGACCCCATCAAAGTGAGG | 189 |
| ABCG5 | XM_003472925.3 | ATCCTGAGGCTGCTCGATTT | CCAGATCCAATCAGCAACCC | 163 |
| ABCG8 | XM_003472926.2 | GTCTCAACTCCCACCCTCTC | CTGAAGGGTCTTCTCCGAGG | 170 |
| CYP7a1 | GQ507494.1 | CTGGAGAAGGCAGGTCAACA | CTCCTTAGCTGTCCGGATGT | 150 |
| IL-8 | NM_001173399.2 | GGCAGCCTTCCTGCTCTCT | CAGCTCCGAGACCAACTTTGT | 67 |
| MCP-1 | NM_001172926.1 | TGCCAAACTGGACCAGAGAA | CGAATGTTCAAAGGCTTTGAAGT | 75 |
| TNF-α | NM_001173025.1 | GCCGTCTCCTACCCGGAAAA | TAGATCTGCCCGGAATCGGC | 203 |
| TGF-β | NM_001173023.1 | AACCCGAGCCGGACTACTATG | TGCTTTTATAGATATTGTGGCTGT TGT | 78 |
| Col1a1 | XM_003466865.2 | CTGGACAGCGTGGTGTAGTC | TCCAGAAGGACCTTGTTTGC | 104 |
| α-sma | ENSCPOT00000011693.2 | GACATCAAGGAGAAGCTGTG | GCTGTTGTAGGTGGTTTCAT | 273 |
HMGR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; LDLR, low-density lipoprotein receptor; SREBP2, sterol regulatory element binding protein 2; ABCG5/8, ATP-binding cassette sub-family G member 5/8; CYP7a1, cytochrome P450 7A1; IL-8, interleukin 8; MCP-1, monocyte chemotactic protein 1; TNF-α, tumor necrosis factor α; TGF-β, transforming growth factor β; Col1a1, collagen 1a1; α-sma, α smooth muscle actin.