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. 2019 Nov 2;11(11):1018. doi: 10.3390/v11111018

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NoV cross-blocking antibodies after monovalent NoV GII.17 VLP immunization. Termination sera was pooled and serially 2-fold diluted starting at a 1:20 dilution and analyzed in a blocking assay against seven heterologous GI and GII VLPs. The blocking index (%) was calculated as [100% − [OD (wells with serum)/OD(wells without serum, maximum VLP binding)] × 100%] and shown are the blocking index (%) of the two analysis. The horizontal dashed line represents a blocking titer of 50% (BT50).