Table 2.
Analysis of Variance for body composition and metabolic data in HFD-fed female and male Sdc4-/- and WT mice.
| Phenotype | Source a | Df b | MS c | F d | P-Value | Means ± SE |
|---|---|---|---|---|---|---|
| %BFM | Sex | 1 | 28.27 | 2.37 | 0.1377 | F: 44.91 ± 2.04; M: 45.98 ± 0.41 |
| Genotype | 1 | 290.98 | 24.43 | <0.0001 | WT: 42.20 ± 1.48; KO: 48.31 ± 0.60 | |
| Genotype x Sex | 1 | 210.86 | 17.70 | 0.0004 | WTF: 37.62 ± 2.22; KOF: 50.13 ± 1.75 WTM: 45.48 ± 0.54; KOM: 46.48 ± 0.74 |
|
| Error | 22 | 4.05 | ||||
| %LBM | Sex | 1 | 8.06 | 0.84 | 0.3691 | F: 51.57 ± 2.04; M: 51.37 ± 0.41 |
| Genotype | 1 | 246.52 | 25.72 | <0.0001 | WT: 54.55 ± 1.19; KO: 48.82 ± 0.57 | |
| Genotype x Sex | 1 | 152.68 | 15.93 | 0.0006 | WTF: 58.06 ± 1.91; KOF: 46.94 ± 1.64 WTM: 52.03 ± 0.44; KOM: 50.71 ± 0.64 |
|
| Error | 22 | 9.59 | ||||
| TC (mg/dL) | Sex | 1 | 37,771.05 | 100.23 | <0.0001 | F: 74.54 ± 5.91; M: 155.35 ± 7.21 |
| Genotype | 1 | 497.05 | 1.32 | 0.2650 | WT: 114.80 ± 15.85; KO: 119.36 ± 10.97 | |
| Genotype x Sex | 1 | 3165.53 | 8.40 | 0.0092 | WTF: 57.61 ± 1.69; KOF: 90.48 ± 5.29 WTM: 162.45 ± 6.33; KOM: 148.24 ± 12.99 |
|
| Error | 19 | 376.86 | ||||
| TG (mg/dL) | Sex | 1 | 1374.67 | 4.89 | 0.0401 | F: 61.98 ± 5.78; M: 74.60 ± 5.90 |
| Genotype | 1 | 1534.92 | 5.46 | 0.0312 | WT: 61.44. ± 5.85; KO:75.05 ± 5.75 | |
| Genotype x Sex | 1 | 1627.66 | 5.80 | 0.0270 | WTF: 41.32 ± 2.09; KOF: 75.76 ± 3.62 WTM: 74.85 ± 4.56; KOM: 74.34 ± 11.51 |
|
| Error | 18 | 280.87 | ||||
| Insulin (ng/dL) | Sex | 1 | 15.21 | 188.34 | <0.0001 | F: 1.39 ± 0.07; M: 7.39 ± 0.87 |
| Genotype | 1 | 0.01 | 0.09 | 0.7648 | WT: 4.94 ± 1.33; KO:4.13 ± 0.91 | |
| Genotype x Sex | 1 | 0.23 | 2.81 | 0.1101 | WTF: 1.22 ± 0.09; KOF: 1.53 ± 0.07 WTM: 8.05 ± 1.49; KOM: 6.73 ± 0.97 |
|
| Error | 19 | 0.08 | ||||
| Glucose(mg/dL) | Sex | 1 | 0.20 | 7.61 | 0.0125 | F:185.82 ± 12.24; M: 217.75 ± 11.43 |
| Genotype | 1 | 0.02 | 0.87 | 0.3636 | WT: 202.18 ± 18.00; KO: 202.75 ± 5.46 | |
| Genotype x Sex | 1 | 0.43 | 16.61 | 0.0006 | WTF: 155.2 ± 19.23; KOF: 211.33 ± 4.07 WTM: 241.33 ± 16.36; KOM: 194.34 ± 9.23 |
|
| Error | 19 | 0.026 | ||||
| ISI | Sex | 1 | 20.60 | 294.96 | <0.0001 | F: 32.40 ± 4.49; M: 4.86 ± 0.55 |
| Genotype | 1 | 0.24 | 3.40 | 0.0810 | WT: 22.90 ± 6.95; KO: 13.57 ± 2.45 | |
| Genotype x Sex | 1 | 1.57 | 22.41 | 0.0001 | WTF: 45.57 ± 5.47; KOF: 21.43 ± 0.91 WTM: 4.01 ± 0.36; KOM: 5.71 ± 0.97 |
|
| Error | 19 | 0.07 |
a Source of variation. b Degrees of freedom. c Mean Squares computed from Type III Sums of Squares. d F-statistics based on the ratio of Mean Squares. SE: Standard Error. %BFM: Percent body fat mass. %LBM; Percent lean body mass. TC: Total cholesterol. TG: Triglycerides. ISI: Insulin sensitivity index. F: Female. M: Male. KO: Sdc4-/- . WTF: Wild-type female; KOF: Sdc4-/- female. WTM: Wild-type male. KOM: Sdc4-/- male. Insulin, glucose, and ISI data were log10 transformed to fulfill the assumption of normality.