Table 1.

L-expression plasmid cloning strategy primer. italics—L sequence; underline—mCherry sequence.

Name	For/Rev	Sequence (5′-3′ Direction)
CD1506insertGGSG for	for	GGCTCAGGCGGTGGATCCGGCCTGTGGCTGTTCAGCGACG
CD1506insertGGSG rev	rev	GCTCCCTCCGCCGCTTCCGCCCTGGCTGTAGTGGCTGCGG
CD1537insertGGSG for	for	GGCTCAGGCGGTGGATCCGGCAGCGGCAAGGACAAGAACGAG
CD1537insertGGSG rev	rev	GCTCCCTCCGCCGCTTCCGCCCAGGAAGGGCTTGTACAGGATC
MT1603insertGGSG for	for	GGCTCAGGCGGTGGATCCGGCGAGAGCCGCGGCACCATCAC
MT1603insertGGSG rev	rev	GCTCCCTCCGCCGCTTCCGCCGCGGCCCCAGGGAGGGTAG
MT1620insertGGSG for	for	GGCTCAGGCGGTGGATCCGGCTACCCCAAGATGCTGGAGATGC
MT1620insertGGSG rev	rev	GCTCCCTCCGCCGCTTCCGCCTGGGGTGGTGGTGTAGTACAC
MT1889insertGGSG for	for	GGCTCAGGCGGTGGATCCGGCCAGTTCATCCCCGACCCCTTC
MT1889insertGGSG rev	rev	GCTCCCTCCGCCGCTTCCGCCGCTGGGGATGCCGGTCAGG
mCherry-GGSG for	for	GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAATGGTGAGCAAGGGCGAGG
mCherry-GGSG rev	rev	GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCCTTGTACAGCTCGTCCATG
Gibson new for	for	GGCAGGGTCGGAACAGGAG
Gibson new rev	rev	CAGGCGTTTCCCCCTGGAAG