Viremia and viral replication in tissues of mice that were infected with mouse-adapted EBOV. Groups of mice (n = 6) were infected intraperitoneally with 10 PFU of either EBOV/Mayinga-MA, EBOV/Makona-MA-PP1, EBOV/Makona-preMA, EBOV/Makona-rgMA, or the two single mutant viruses EBOV/Makona-rgMA-10979 or EBOV/Makona-rgMA-2357. (A) Blood, (B) liver, (C) spleen, and (D) kidney samples were collected on days 3 and 5 post-infection. (E) Viral dissemination to the lung, brain, and intestine was also examined for EBOV/Makona-rgMA infected mice on day 5 post-infection. Mean viral titers are shown, 2-way ANOVA with Bonferroni post-tests was performed, *** p-value < 0.001. The dashed line denotes the limit of detection of the TCID50/mL or TCID50/g assay.