Table 1.
Virulence comparison of mouse-adapted EBOV/Makona viruses to mouse-adapted EBOV-Mayinga. Groups of 4 to 6 week old BALB/c mice (n = 3 to 6) were infected by intraperitoneal injection with either EBOV/Makona-preMA, reverse genetics generated EBOV/Makona-MA (EBOV/Makona-rgMA), EBOV/Makona-MA with either the 2357 mutation (EBOV/Makona-rgMA-2357) or the 10,979 mutation (EBOV/Makona-rgMA-10979), or EBOV/Mayinga-MA with various doses shown in plaque forming units (PFU). Survival and weight loss were monitored for up to 18 days post-infection. Median lethal dose (LD50) was calculated using the Reed and Muench method.
| Virus | Survival at Dose Tested (PFU) | LD50 (PFU) | |||||
| 0.001 | 0.01 | 0.1 | 1 | 10 | 100 | ||
| EBOV/Makona-preMA | 6/6 | >100 | |||||
| EBOV/Makona-rgMA | 6/6 | 1/6 | 2/6 | 0/6 | 0/3 | 0.004 | |
| EBOV/Makona-rgMA-2357 | 3/3 | 3/3 | 3/3 | 3/3 | >100 | ||
| EBOV/Makona-rgMA-10979 | 6/6 | 1/6 | 1/6 | 0/6 | 0/6 | 0/3 | 0.004 |
| PFU | |||||||
| 0.0045 | 0.045 | 0.45 | 4.5 | 45 | 450 | ||
| EBOV/Mayinga-MA | 2/3 | 2/3 | 0/3 | 1/3 | 0/3 | 0/3 | 0.08 |