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SIGIRR-/- cells have an increased NF-κB activation compare with SIGIRR+/+ cells. SIGIRR-/- and SIGIRR+/+ 3D4/2 cells were infected with PCV2 at MOI of 1 for 12 h, and the SIGIRR, p-p65, and p65 expression were measured by Western blotting (A). Densitometric analysis was performed by Image J software and results were normalized to β-actin (B). Results are mean ± SE of three independent experiments. * p < 0.05.
