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Analysis of the cytopathic effect (CPE) and EGFP reporter expression in prM-E-expressing packaging cells transfected with the ZIKV Natal RGN replicon. CPE and EGFP reporter expressions in mock cells (top), packaging cells (middle), and replicon-transfected packaging cells (bottom) were photographed using light and fluorescence microscopes 24 (A) and 72 (B) h post-incubation. Scale bar, 100 μm.
