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Antigenicity and quantitative analysis of ZIKV Natal RGN single-round infectious particles (SRIPs). The antigenicity, relative viral genome levels, and titer of SRIPs collected from the supernatant of the packaging cells transfected with the ZIKV Natal RGN replicon were analyzed using dot blot (A), real-time RT-PCR (B), and TCID50 (C) assays, respectively.
