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. 2019 Nov 19;11(11):2824. doi: 10.3390/nu11112824

Figure 5.

Figure 5

Representative immunoblot images of all proteins studied and their regulatory phosphorylations and the total amount of protein loaded (Reactive Brown staining) from a single participant in the study belonging to the whey protein group. From top to bottom: FoxO1, pSer256 FoxO1, FoxO3, pSer253 FoxO3, MuRF1, MAFbx, eIF3f, AMPKα, pThr172 AMPKα, ULK1, pSer555 ULK1, pSer15 Beclin 1, LC3BI, LC3BII, p62/SQSTM1 and Reactive Brown (as protein loading control). Quadruplicate assays of a non-intervention human sample were included on each gel as a loading control. The various phases of the intervention are explained in Figure 1. PRE, before the intervention; CRE, caloric restriction and exercise; CD, control diet. Estimated molecular weights are indicated by arrows.