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. 2019 Nov 15;11(11):2792. doi: 10.3390/nu11112792

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of polydatin on conjunctival goblet cells in exorbital lacrimal gland-excised rats. (A,B) Histology by Periodic acid-Schiff (PAS) staining of the conjunctival epithelium in exorbital lacrimal gland-excised rats. In the conjunctiva, epithelial cells and subepithelial fibroblasts are seen. PAS positive goblet cells were distributed in the conjunctival epithelium. Bar, 100 µm. mRNA levels of (C) IL-1β, (D) INF-γ, (E) TNF-α, (F) IL-6, and (G) MUC5AC were assessed by real-time PCR assay. GAPDH was considered an internal control. Data are the mean ± SEM of three independent experiments for all groups. # p < 0.05, significantly different from normal mice, * p < 0.05, significantly different from vehicle-treated dry eye mice.