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. 2019 Nov 4;11(11):1024. doi: 10.3390/v11111024

Figure 5.

Figure 5

ZIKV NS5 inhibits IFN-λ1-promoter activation on both IRF3 and NF-ĸB pathways. HEK293 cells were co-transfected with expression plasmids for ∆RIG-I and ZIKV NS3 or NS5 plasmids, and with IFN-λ1-promoter-luciferase reporter plasmids which were mutated on (A) IRF3 or (B) NF-ĸB1 binding sites. An expression plasmid for HCV NS3/4A was used as a control. Luc activity was measured at 24 h after transfection. ** indicate a significant reduction in promoter activation (p < 0.05). All experiments were repeated at least three times and a representative experiment is shown.