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. 2019 Nov 1;11(11):1012. doi: 10.3390/v11111012

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Flow chart showing the numbers of HIV-1 positives samples analyzed and consensus envelope sequences of HIV-1-infected individuals include in the study. From a total of 243 sequences obtained, 95% (231) of subtype B HIV-1 sequences were included in the current study. The non-B HIV-1 subtype envelope sequences representing 8.7% (four for transmitted/founder (TF) and eight for recent (RC) viruses) of the LSPQ serobank HIV-1 positive samples were excluded for subsequent genetic analysis because they may have influenced the results. The HIV-1 envelope sequences of transmitted/founder (TF) viruses derived from acute infection, recent viruses (RC) for early infection and chronic viruses for chronic infection.