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. 2019 Nov 19;11(11):1079. doi: 10.3390/v11111079

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Screening of reactive peptides. (A) Spot blotting of nitrocellulose membranes in which peptides were synthesized. The chart on the left represents the peptide sequence for each spot. Preimmune and YFV sera did not react with any selected peptide, while DENV sera were positive for some peptides. The sequences highlighted in red are reactive for both DENV sera (1, 2, 3 and 4) and were chosen to be used in the following experiments; (B) Indirect IgG ELISA for validation of soluble peptides. Serum samples from infected rabbits were tested on plates with Pep1, Pep2, Pep3 and DENV3E protein (positive control). Both peptides absorbances of anti-dengue sera were higher than the preimmune control. Compared to peptides, protein E is also positive for YFV serum. Red line: Blank control.