Table 3.
Results of the microneutralization assay comparing the usage of lavage samples to serum samples for the animals that had been inoculated with either the Neuddörfl, HM 4-2, HB171/11, or BaWa 15/943 TBEV-Eu strain or Langat virus. In-contact animals are shaded in grey. ND50: 50% neutralizing dose.
| HM 4-2 | BaWa 15/943 | Neudörfl | ||||||
| ID | serum | lavage | ID | serum | lavage | ID | serum | lavage |
| ND50 | ND50 | ND50 | ND50 | ND50 | ND50 | |||
| 2-1 | 1:960 | 1:5 | 24-1 | 1:480 | 1:10 | 1-1 | 1:1280 | 1:5 |
| 2-2 | 1:240 | neg. 1 | 24-3 | 1:240 | 1:5 | 1-2 | 1:640 | 1:10 |
| 2-3 | 1:1280 | 1:7.5 | 24-4 | 1:320 | 1:20 | 1-3 | 1:240 | 1:5 |
| 2-4 | neg. 1 | neg. 1 | 24-2 | neg. 1 | neg. 1 | 1-4 | neg.1 | neg. 1 |
| 3-1 | 1:1280 | 1:40 | 25-1 | 1:80 | 1:5 | 6-1 | 1:960 | 1:10 |
| 3-2 | 1:1280 | 1:40 | 25-2 | 1:120 | 1:5 | 6-2 | 1:1280 | 1:7.5 |
| 3-3 | 1:1280 | 1:10 | 25-3 | 1:160 | 1:15 | 6-3 | 1:1280 | 1:30 |
| 3-4 | neg. 1 | neg. 1 | 25-4 | neg. 1 | neg. 1 | 6-4 | neg. 1 | neg. 1 |
| HB 171/11 | Langat virus | |||||||
| ID | serum | lavage | ID | serum | lavage | |||
| ND50 | ND50 | ND50 | ND50 | |||||
| 4-1 | 1:640 | 1:10 | 26-1 | 1:120 | neg. 1 | |||
| 4-2 | 1:1280 | 1:40 | 26-2 | 1:160 | 1:2.5 | |||
| 4-3 | 1:640 | 1:5 | 26-3 | 1:240 | 1:2.5 | |||
| 4-4 | neg. 1 | neg. 1 | 26-4 | neg. 1 | neg. 1 | |||
| 5-1 | 1:160 | 1:2.5 | 27-1 | 1:160 | neg. 1 | |||
| 5-2 | 1:1280 | 1:5 | 27-3 | 1:320 | 1:10 | |||
| 5-3 | 1:1280 | 1:40 | 27-4 | 1:320 | 1:7.5 | |||
| 5-4 | neg. 1 | neg. 1 | 27-2 | neg. 1 | neg. 1 | |||
1 neg. stands for a detection limit of <1:20 for serum samples and <1:5 for lavage samples.