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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: SLAS Discov. 2019 May 30;24(8):802–816. doi: 10.1177/2472555219849838

Figure 1.

Figure 1.

High-throughput flow cytometry screen for modulators of HP1-mediated heterochromatin formation. (a) Cartoon of the CiA:Oct4 system utilizing CIP to recruit csHP1α. Addition of rapamycin facilitated the bridging of Gal4-FKBP and FRB-csHP1α fusions by CIP, resulting in HP1-heterochromatin formation and gene repression. (b) Diagram outlining the primary screening strategy workflow over a 3-day experimental time course. (c) Representative histogram of GFP fluorescence intensity for top inhibitors of HP1-mediated repression (UNC2524 and UNC617) at 10 μM compared with ±6 nM rapamycin controls. (d) Results of small-molecule screen showing the percentage of GFP-positive populations after 48 h of CIP-mediated HPI recruitment. Inset boxes highlight inhibitors or enhancers of HP1-mediated gene repression.