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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Sci Signal. 2019 Mar 19;12(573):eaau4604. doi: 10.1126/scisignal.aau4604

Fig. 1. HIPK2 is critical for antiviral immunity in vivo.

Fig. 1.

(A) Survival analysis of age-and sex-matched wild-type (WT) and Hipk2+/− mice inoculated with VSV. Data are from a total of eight mice per group from three experiments. d, days. (B to E) qRT-PCR analysis of (B) Ifn-β, (C) Isg15, (D) VSV glycoprotein (VSV-G), and (E) Ifng mRNA expression in blood cells from mice at the indicated times after infection with VSV. Data with means ± SEM of six mice per group from three experiments. NS, not significant. (F to H) qRT-PCR analysis of (F) Ifn-β, (G) Isg15, or (H) VSV-G mRNA expression in wild-type, Hipk2+/−, or Hipk2−/− peritoneal macrophages infected with VSV for 24 hours. MOI, multiplicity of infection. (I) Survival analysis of age-and sex-matched wild-type and Hipk2+/− mice treated with antibody against IFNAR and infected with VSV. Data are from a total of 10 mice per group from three experiments. IgG, immunoglobulin G. (J) Viral load in the blood after wild-type and Hipk2+/− mice were treated with antibody against IFNAR and infected with VSV. Data with means ± SEM are from three experiments. *P < 0.05 and **P < 0.01 by log-rank Mantel-Cox test (A and I) or an unpaired two-tailed nonparametric Mann-Whitney U test (B to E and J).