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. Author manuscript; available in PMC: 2020 Apr 16.
Published in final edited form as: Nature. 2019 Oct 16;574(7778):359–364. doi: 10.1038/s41586-019-1647-8

Extended Data Figure 4. Neural excitation, neuropeptide signaling and lifespan in C. elegans.

Extended Data Figure 4.

a, Increased excitation of ASH neurons following RNAi against the GABA vesicular transporter unc-47. GCaMP imaging was performed on worms with enhanced neuronal RNAi (See Figure 3 legend and Methods for details) for unc-47 (n=37) or controls (n=43) at day 2. **P= 6.8e-3 by the Mann–Whitney U test. b, RNAi for unc-47 reduces lifespan. Worms with enhanced neuronal RNAi were treated with unc-47 (n=31) or control RNAi (n=84). Shown is a representative lifespan analysis replicated 3 times. P= 1.3e-6 by log-rank test. c, Reduction of synaptic neurotransmission or neuropeptide signaling extend lifespan in C. elegans. Mutations in genes affecting glutamatergic neurotransmission (eat-4), presynaptic function (unc-13) and neuropeptide signaling (egl-3) exhibit comparable lifespan extension. WT, n=57; eat-4(nj2), n=54. P≤2.2e-16; unc-13(e51), n=92, P=3.6e-14; egl-3(gk238), n=35, P=8.3e-11 by log-rank test. Shown are curves representative of two independent replicates. d, Extension of lifespan by egl-3 RNAi in worms with enhanced neuronal RNAi. Shown are lifespan curves representative of two independent replicates. egl-3 RNAi (n=47 worms); Empty Vector (n=84 worms). P=3.5e-11 by the log-rank test.