Supplemental Figure S1.

CD31⁺ lymphatic vessels (LVs) are stained positively for podoplanin (PDPN). Whole-mount triple fluorescent staining was performed on the ear skin of wild-type mice with fluorescein isothiocyanate–anti–α-smooth muscle actin (α-SMA; green), phycoerythrin (PE)–anti-CD31 (red), and hamster–anti-PDPN, followed by Dylight–anti-hamster (blue). A: Representative images showing a large LV with valve (arrowhead) that is stained positively for CD31 and PDPN and partially for α-SMA. B: Smooth muscle cell (SMC) coverage in CD31⁺ LVs and SMC coverage in PDPN⁺ LVs. Unpaired t-test was performed. C: Correlation analysis of SMC coverage in CD31⁺ LVs and in PDPN⁺ LVs. Data are expressed as means ± SD (B). n = 5 mice (B). Scale bar = 50 μm (A).