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. 2019 Dec;189(12):2516–2530. doi: 10.1016/j.ajpath.2019.08.009

Figure 4.

Figure 4

Impaired draining function of mature lymphatic vessels (LVs) in Relb−/− mice. Near-infrared indocyanine green (NIR-ICG) lymphatic imaging was used in Relb−/− and control mice after injection of ICG intradermally into footpads. A: Representative NIR-ICG images showing mature LVs (red arrows, blue circle), upstream popliteal lymph node (PLN; yellow arrows), footpad area (black arrows), and region of interest (ROI) where the pulses were measured (red circle indicates ROI at collateral LVs that bypass the PLN). Marked ICG leakage (green arrows) is observed in a Relb−/− mouse leg. B: ICG signals in a dominant LV (blue) of one control mouse and a dominant LV (green) and a collateral LV (red) of one Relb−/− mice. C: Lymphatic pulses/minute of dominant LV. Unpaired t-test was performed. Data are expressed as means ± SD (C). n = 5 control mice (10 legs; C); n = 4 Relb−/− mice (8 legs; C). *P < 0.05 versus control mice.