Skip to main content
. 2019 Dec;189(12):2516–2530. doi: 10.1016/j.ajpath.2019.08.009

Figure 6.

Figure 6

Functional changes in lymphatic endothelial cells (LECs) with RelB knocked down. LECs were infected with lentiviruses containing shRNA against RelB or control shRNA. A: The expression levels of RelB by real-time quantitative PCR and Western blot analysis. B: Growth curves of LECs by MTT assays. Unpaired t-test was performed. The experiment was repeated three times. C: Cells were stained with annexin V–phycoerythrin (V-PE)/7–aminoactinomycin D (7-AAD) and analyzed by flow cytometry. The percentage of late (annexin V+/7-AAD+) apoptotic cells is shown in the upper right corner and early (annexin V+/7-AAD-) apoptotic cells is shown in the lower right corner. D: Cell migration by transwell assays. Unpaired t-test was performed. The experiment was repeated two times. E: Adhesion was examined by co-culturing eFluor 670–labeled lymphatic smooth muscle cells (LSMCs) and green fluorescent protein (GFP)–labeled LECs for 1 hour, washing off floating cells, and then analyzing the number of GFP+ LECs by flow cytometry. The percentage of GFP+ LECs per total cells is given. Unpaired t-test was performed. The experiment was repeated three times. Data are expressed as means ± SD (A, B, D, and E). n = 12 wells (A and B). n = 3 wells (D and E). *P < 0.05 versus control shRNA. Original magnification, ×4 (D).