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Agarose gel electrophoretogram and sequence output of ARMS PCR product for AcrR. All representative 5 isolates showed positive result for the presence of mutation (A). Normal AcrR has cytosine (B)19 and mutant AcrR sequence has thymine (C) at position 133.
L indicates the DNA ladder; ARMS PCR, amplification refractory mutation system polymerase chain reaction. Escherichia coli isolate numbers are indicated above the image of the gel with PCR products ~800 and 500 bp.
