Fig. 2.

Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference.

DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; GAPDH=glyceraldehyde-3-phosphate dehydrogenase