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. 2019 Sep 17;33(12):13398–13411. doi: 10.1096/fj.201901366R

Figure 5.

Figure 5

ROS regulation of IDH2-directed tumor cell motility. A) PC3 cells transfected with siCtrl or siIDH2 were reconstituted with Prx3 or loss-of-function Cys108Ser (C108S) Prx3 mutant (Prx3-Mut) and quantified for speed of mitochondrial movements (top, n = 85–92) or distance traveled by individual mitochondria (bottom, n = 85–92). ***P < 0.0001. B) The conditions are as in A, and reconstituted PC3 cells were analyzed for cell motility in 2D contour plots. Each tracing corresponds to the movement of an individual cell. The cutoff velocities for slow-moving or fast-moving (<0.39 μm/min or >0.39 μm/min, respectively) cells are shown. Representative experiment (n = 3). C, D) PC3 cells transfected with siCtrl or siIDH2 and reconstituted as in A were analyzed for cell migration [top n = 10–14 (C)] or Matrigel invasion [bottom n = 10–12 (C)] or Western blotting (D). Means ± sd. ***P < 0.0001. E) PC3 cells transfected with siCtrl or siIDH2 were incubated with the ROS scavenger, MnTBAP, and analyzed by Western blotting. Ns, not significant; p, phosphorylated.