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. 2019 Oct 30;33(12):14588–14601. doi: 10.1096/fj.201901646R

Figure 1.

Figure 1

AdipoR1 and AdipoR2 are expressed in human uterine smooth muscle. A) mRNA for AdipoR1and AdipoR2 is detected in NP (n = 6) and pregnant (n = 7) human uterus. Data are means ± sem for fold difference in expression of the target gene relative to an adipose tissue calibrator using RPL13A as the reference gene. **P < 0.01 vs. NP AdipoR1, ##P < 0.01 vs. NP AdipoR2. B) AdipoR1 (top panels) and AdipoR2 (bottom panels) protein is detected in NP (n = 5) and pregnant (n = 6) human uterine smooth muscle. Tissue was prepared as total lysate (Total) or as crude membrane fraction (Memb) with β-actin as loading reference. Antibody specificity was confirmed using myc-tagged expression constructs (Supplemental Fig. S2).